TP構造ギャラリー
3VLF
PDB ID | 3VLF | |
Protein | Hsm3-Rpt1 complex | |
Comment | ||
PubMed ID | 22334676 | |
Authors | Takagi K, Kim S, Yukii H, Ueno M, Morishita R, Endo Y, Kato K, Tanaka K, Saeki Y, Mizushima T. | |
Title | Structural basis for specific recognition of Rpt1p, an ATPase subunit of 26 S proteasome, by proteasome-dedicated chaperone Hsm3p. | |
Method | X-ray diffraction | |
Resolution | 3.80 |
分子情報[1]
Protein | Hsm3 |
Organism | Saccharomyces cerevisiae |
Uniprot | P38348 |
Sequence | Se-Met protein |
Ligand | |
Vector | pET-28a |
Expression host | Escherichia coli (B834(DE3)) |
Cell culture | |
Protein purification | Ni-NTA, Q Sepharose, Superdex 75 |
分子情報[2]
Protein | Rpt1 |
Organism | Saccharomyces cerevisiae |
Uniprot | P33299 |
Sequence | Se-Met protein, C-terminal domain(381-467) |
Ligand | |
Vector | pET-21 |
Expression host | Escherichia coli (B834(DE3)) |
Cell culture | |
Protein purification | Ni-NTA, Q Sepharose, Superdex 75 |
>>一覧に戻る