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TP構造ギャラリー

3VLF

3VLF PDB ID 3VLF
Protein Hsm3-Rpt1 complex
Comment
PubMed ID 22334676
Authors Takagi K, Kim S, Yukii H, Ueno M, Morishita R, Endo Y, Kato K, Tanaka K, Saeki Y, Mizushima T.
Title Structural basis for specific recognition of Rpt1p, an ATPase subunit of 26 S proteasome, by proteasome-dedicated chaperone Hsm3p.
Method X-ray diffraction
Resolution 3.80

分子情報[1]

Protein Hsm3
Organism Saccharomyces cerevisiae
Uniprot P38348
Sequence Se-Met protein
Ligand
Vector pET-28a
Expression host Escherichia coli (B834(DE3))
Cell culture
Protein purification Ni-NTA, Q Sepharose, Superdex 75

分子情報[2]

Protein Rpt1
Organism Saccharomyces cerevisiae
Uniprot P33299
Sequence Se-Met protein, C-terminal domain(381-467)
Ligand
Vector pET-21
Expression host Escherichia coli (B834(DE3))
Cell culture
Protein purification Ni-NTA, Q Sepharose, Superdex 75

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