TP構造ギャラリー
3VH3
PDB ID | 3VH3 | |
Protein | Atg7-Atg8 complex | |
Comment | Atg7(295-630)-Atg8(K26P) | |
PubMed ID | 22055191 | |
Authors | Noda NN, Satoo K, Fujioka Y, Kumeta H, Ogura K, Nakatogawa H, Ohsumi Y, Inagaki F. | |
Title | Structural basis of Atg8 activation by a homodimeric E1, Atg7. | |
Method | X-ray diffraction | |
Resolution | 2.00 |
分子情報[1]
Protein | Atg7 |
Organism | Saccharomyces cerevisiae |
Uniprot | P38862 |
Sequence | C-terminal domain(295-630) |
Ligand | Zn |
Vector | pACYCDuet-1 |
Expression host | Escherichia coli (BL21(DE3)) |
Cell culture | |
Protein purification | GST affinity, Ni-chelating, gel filtration |
分子情報[2]
Protein | Atg8 |
Organism | Saccharomyces cerevisiae |
Uniprot | P38182 |
Sequence | K26P |
Ligand | |
Vector | pGEX-6P-1 |
Expression host | Escherichia coli (BL21(DE3)) |
Cell culture | |
Protein purification | GST affinity, Ni-chelating, gel filtration |
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