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TP構造ギャラリー

3H3S

3H3S PDB ID 3H3S
Protein CERT START domain
Comment HPA-15 complex
PubMed ID 20036255
Authors Kudo N, Kumagai K, Matsubara R, Kobayashi S, Hanada K, Wakatsuki S, Kato R.
Title Crystal structures of the CERT START domain with inhibitors provide insights into the mechanism of ceramide transfer.
Method X-ray diffraction
Resolution 1.75

分子情報[1]

Protein CERT
Organism Homo sapiens
Uniprot Q9Y5P4
Sequence START domain(347-598)
Ligand HPA-15
Vector pGEX-5T-1
Expression host Escherichia coli (DH5alpha)
Cell culture induction with 0.1 mM IPTG, harvest 20 h at 30 deg C post induction.
Protein purification Glutathione Sepharose 4B, Ni-NTA, Mono Q, Superdex 75

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