TP構造ギャラリー
3H3R
PDB ID | 3H3R | |
Protein | CERT START domain | |
Comment | HPA-14 complex | |
PubMed ID | 20036255 | |
Authors | Kudo N, Kumagai K, Matsubara R, Kobayashi S, Hanada K, Wakatsuki S, Kato R. | |
Title | Crystal structures of the CERT START domain with inhibitors provide insights into the mechanism of ceramide transfer. | |
Method | X-ray diffraction | |
Resolution | 1.85 |
分子情報[1]
Protein | CERT |
Organism | Homo sapiens |
Uniprot | Q9Y5P4 |
Sequence | START domain(347-598) |
Ligand | HPA-14 |
Vector | pGEX-5T-1 |
Expression host | Escherichia coli (DH5alpha) |
Cell culture | induction with 0.1 mM IPTG, harvest 20 h at 30 deg C post induction. |
Protein purification | Glutathione Sepharose 4B, Ni-NTA, Mono Q, Superdex 75 |
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