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TP構造ギャラリー

3AQP

3AQP PDB ID 3AQP
Protein SecDF
Comment
PubMed ID 21562494
Authors Tsukazaki T, Mori H, Echizen Y, Ishitani R, Fukai S, Tanaka T, Perederina A, Vassylyev DG, Kohno T, Maturana AD, Ito K, Nureki O.
Title Structure and function of a membrane component SecDF that enhances protein export.
Method X-ray diffraction
Resolution 3.3

分子情報[1]

Protein SecDF
Organism Thermus thermophilus
Uniprot Q5SKE6
Sequence N2D
Ligand
Vector pTV118N
Expression host Escherichia coli (AD202)
Cell culture growth at 37 deg C, induction with 1 mM IPTG, harvest 12 h at 37 deg C post induction.
Protein purification Ni-NTA, HiTrap desalting, HiTrap SP, Superose 6

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