TP構造ギャラリー
3AQO
PDB ID | 3AQO | |
Protein | SecDF | |
Comment | P1 domain | |
PubMed ID | 21562494 | |
Authors | Tsukazaki T, Mori H, Echizen Y, Ishitani R, Fukai S, Tanaka T, Perederina A, Vassylyev DG, Kohno T, Maturana AD, Ito K, Nureki O. | |
Title | Structure and function of a membrane component SecDF that enhances protein export. | |
Method | X-ray diffraction | |
Resolution | 2.6 |
分子情報[1]
Protein | SecDF |
Organism | Thermus thermophilus |
Uniprot | Q5SKE6 |
Sequence | P1 domain(35-263) |
Ligand | |
Vector | pET-26b |
Expression host | Escherichia coli (BL21-CodonPlus(DE3)-RIL) |
Cell culture | growth at 37 deg C, induction with 1 mM IPTG, harvest 4 h at 37 deg C post induction. |
Protein purification | TALON, NAP-5, Ni-NTA Agarose, Benzamidine, Mono Q, Superdex 200 |
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