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TP構造ギャラリー

3AQO

3AQO PDB ID 3AQO
Protein SecDF
Comment P1 domain
PubMed ID 21562494
Authors Tsukazaki T, Mori H, Echizen Y, Ishitani R, Fukai S, Tanaka T, Perederina A, Vassylyev DG, Kohno T, Maturana AD, Ito K, Nureki O.
Title Structure and function of a membrane component SecDF that enhances protein export.
Method X-ray diffraction
Resolution 2.6

分子情報[1]

Protein SecDF
Organism Thermus thermophilus
Uniprot Q5SKE6
Sequence P1 domain(35-263)
Ligand
Vector pET-26b
Expression host Escherichia coli (BL21-CodonPlus(DE3)-RIL)
Cell culture growth at 37 deg C, induction with 1 mM IPTG, harvest 4 h at 37 deg C post induction.
Protein purification TALON, NAP-5, Ni-NTA Agarose, Benzamidine, Mono Q, Superdex 200

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