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TP構造ギャラリー

3APM

3APM PDB ID 3APM
Protein PAD4
Comment SNP(S55G, A82V, A112G)
PubMed ID 21245532
Authors Horikoshi N, Tachiwana H, Saito K, Osakabe A, Sato M, Yamada M, Akashi S, Nishimura Y, Kagawa W, Kurumizaka H.
Title Structural and biochemical analyses of the human PAD4 variant encoded by a functional haplotype gene.
Method X-ray diffraction
Resolution 2.5

分子情報[1]

Protein PAD4
Organism Homo sapiens
Uniprot Q9UM07
Sequence S55G, A82V, A112G
Ligand
Vector pET-15b
Expression host Escherichia coli (BL21(DE3))
Cell culture growth to OD600= 0.4 at 25 deg C, induction with 0.5 mM IPTG, harvest 15 h at 25 deg C post induction.
Protein purification Ni-NTA Agarose, Mono Q

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