TP構造ギャラリー
3APM
PDB ID | 3APM | |
Protein | PAD4 | |
Comment | SNP(S55G, A82V, A112G) | |
PubMed ID | 21245532 | |
Authors | Horikoshi N, Tachiwana H, Saito K, Osakabe A, Sato M, Yamada M, Akashi S, Nishimura Y, Kagawa W, Kurumizaka H. | |
Title | Structural and biochemical analyses of the human PAD4 variant encoded by a functional haplotype gene. | |
Method | X-ray diffraction | |
Resolution | 2.5 |
分子情報[1]
Protein | PAD4 |
Organism | Homo sapiens |
Uniprot | Q9UM07 |
Sequence | S55G, A82V, A112G |
Ligand | |
Vector | pET-15b |
Expression host | Escherichia coli (BL21(DE3)) |
Cell culture | growth to OD600= 0.4 at 25 deg C, induction with 0.5 mM IPTG, harvest 15 h at 25 deg C post induction. |
Protein purification | Ni-NTA Agarose, Mono Q |
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