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TP構造ギャラリー

3ACP

3ACP PDB ID 3ACP
Protein Rpn14
Comment
PubMed ID 20236927
Authors Kim S, Saeki Y, Fukunaga K, Suzuki A, Takagi K, Yamane T, Tanaka K, Mizushima T, Kato K.
Title Crystal structure of yeast rpn14, a chaperone of the 19S regulatory particle of the proteasome.
Method X-ray diffraction
Resolution 2.0

分子情報[1]

Protein Rpn14
Organism Saccharomyces cerevisiae
Uniprot P53196
Sequence
Ligand
Vector pET-28(+)
Expression host Escherichia coli (BL21(DE3))
Cell culture growth at 37 deg C, induction with 0.1 mM IPTG, harvest 20 h at 18 deg C post induction.
Protein purification Ni-affinity, anion exchange, gel filtration

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