TP構造ギャラリー
3ACP
PDB ID | 3ACP | |
Protein | Rpn14 | |
Comment | ||
PubMed ID | 20236927 | |
Authors | Kim S, Saeki Y, Fukunaga K, Suzuki A, Takagi K, Yamane T, Tanaka K, Mizushima T, Kato K. | |
Title | Crystal structure of yeast rpn14, a chaperone of the 19S regulatory particle of the proteasome. | |
Method | X-ray diffraction | |
Resolution | 2.0 |
分子情報[1]
Protein | Rpn14 |
Organism | Saccharomyces cerevisiae |
Uniprot | P53196 |
Sequence | |
Ligand | |
Vector | pET-28(+) |
Expression host | Escherichia coli (BL21(DE3)) |
Cell culture | growth at 37 deg C, induction with 0.1 mM IPTG, harvest 20 h at 18 deg C post induction. |
Protein purification | Ni-affinity, anion exchange, gel filtration |
>>一覧に戻る