TP構造ギャラリー
2ZPN
PDB ID | 2ZPN | |
Protein | Atg8-Atg19(peptide) complex | |
Comment | ||
PubMed ID | 19021777 | |
Authors | Noda NN, Kumeta H, Nakatogawa H, Satoo K, Adachi W, Ishii J, Fujioka Y, Ohsumi Y, Inagaki F. | |
Title | Structural basis of target recognition by Atg8/LC3 during selective autophagy. | |
Method | X-ray diffraction | |
Resolution | 2.7 |
分子情報[1]
Protein | Atg19 |
Organism | Saccharomyces cerevisiae |
Uniprot | P35193 |
Sequence | 412-415 |
Ligand | |
Vector | |
Expression host | Synthetic construct |
Cell culture | |
Protein purification |
分子情報[2]
Protein | Atg8 |
Organism | Saccharomyces cerevisiae |
Uniprot | P38182 |
Sequence | |
Ligand | |
Vector | pHT1(modified pET-28a(+)) |
Expression host | Escherichia coli(BL21(DE3)) |
Cell culture | |
Protein purification | Ni-NTA, HiTrap CM |
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