TP構造ギャラリー
2ZJS
PDB ID | 2ZJS | |
Protein | SecYE | |
Comment | +Fab complex | |
PubMed ID | 18923527 | |
Authors | Tsukazaki T, Mori H, Fukai S, Ishitani R, Mori T, Dohmae N, Perederina A, Sugita Y, Vassylyev DG, Ito K, Nureki O. | |
Title | Conformational transition of Sec machinery inferred from bacterial SecYE structures. | |
Method | X-ray diffraction | |
Resolution | 3.2 |
分子情報[1]
Protein | Fab56 heavy chain |
Organism | Mus musculus |
Uniprot | |
Sequence | |
Ligand | |
Vector | |
Expression host | |
Cell culture | |
Protein purification |
分子情報[2]
Protein | Fab56 light chain |
Organism | Mus musculus |
Uniprot | |
Sequence | |
Ligand | |
Vector | |
Expression host | |
Cell culture | |
Protein purification |
分子情報[3]
Protein | SecE |
Organism | Thermus thermophilus |
Uniprot | P38383 |
Sequence | |
Ligand | |
Vector | pSTD343 |
Expression host | Escherichia coli (AD202) |
Cell culture | grown at 40 deg C overnight |
Protein purification | Ni-NTA, HiLoad 16/60 Superdex 200 pg, HiTrap SP |
分子情報[4]
Protein | SecY |
Organism | Thermus thermophilus |
Uniprot | Q5SHQ8 |
Sequence | 1-434, L2V, R252G |
Ligand | |
Vector | pTT159 |
Expression host | Escherichia coli (AD202) |
Cell culture | grown at 40 deg C overnight |
Protein purification | Ni-NTA, HiLoad 16/60 Superdex 200 pg, HiTrap SP |
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