研究課題
技術開発研究課題は右カラムarrow

TP構造ギャラリー

2ZAI

2ZAI PDB ID 2ZAI
Protein STT3(AglB) soluble domain
Comment
PubMed ID 18046457
Authors Igura M, Maita N, Kamishikiryo J, Yamada M, Obita T, Maenaka K, Kohda D.
Title Structure-guided identification of a new catalytic motif of oligosaccharyltransferase.
Method X-ray diffraction
Resolution 2.7

分子情報[1]

Protein STT3
Organism Pyrococcus Furiosus
Uniprot Q8U4D2
Sequence soluble domain(471-967)
Ligand
Vector pET-21d
Expression host Escherichia coli (BL21-CodonPlus(DE3)-RIL)
Cell culture growth to OD600= 0.6 at 37 deg C, induction with 0.5 mM IPTG, harvest 4 h at 37 deg C or overnight at 16 deg C post induction.
Protein purification Q Sepharose FF, Superdex 200, RESOURCE Q

>>一覧に戻る

ページトップへ