TP構造ギャラリー
2ZAG
PDB ID | 2ZAG | |
Protein | STT3(AglB) soluble domain | |
Comment | Se-Met protein | |
PubMed ID | 18046457 | |
Authors | Igura M, Maita N, Kamishikiryo J, Yamada M, Obita T, Maenaka K, Kohda D. | |
Title | Structure-guided identification of a new catalytic motif of oligosaccharyltransferase. | |
Method | X-ray diffraction | |
Resolution | 3.0 |
分子情報[1]
Protein | STT3 |
Organism | Pyrococcus Furiosus |
Uniprot | Q8U4D2 |
Sequence | Se-Met protein, soluble domain(471-967) |
Ligand | |
Vector | pET-21d |
Expression host | Escherichia coli (BL21-CodonPlus(DE3)-RIL) |
Cell culture | growth to OD600= 0.6 at 37 deg C, induction with 0.5 mM IPTG, harvest 4 h at 37 deg C or overnight at 16 deg C post induction. |
Protein purification | Q Sepharose FF, Superdex 200, RESOURCE Q |
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