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TP構造ギャラリー

2ZAG

2ZAG PDB ID 2ZAG
Protein STT3(AglB) soluble domain
Comment Se-Met protein
PubMed ID 18046457
Authors Igura M, Maita N, Kamishikiryo J, Yamada M, Obita T, Maenaka K, Kohda D.
Title Structure-guided identification of a new catalytic motif of oligosaccharyltransferase.
Method X-ray diffraction
Resolution 3.0

分子情報[1]

Protein STT3
Organism Pyrococcus Furiosus
Uniprot Q8U4D2
Sequence Se-Met protein, soluble domain(471-967)
Ligand
Vector pET-21d
Expression host Escherichia coli (BL21-CodonPlus(DE3)-RIL)
Cell culture growth to OD600= 0.6 at 37 deg C, induction with 0.5 mM IPTG, harvest 4 h at 37 deg C or overnight at 16 deg C post induction.
Protein purification Q Sepharose FF, Superdex 200, RESOURCE Q

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