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TP構造ギャラリー

2RRN

2RRN PDB ID 2RRN
Protein SecDF
Comment P4 domain
PubMed ID 21562494
Authors Tsukazaki T, Mori H, Echizen Y, Ishitani R, Fukai S, Tanaka T, Perederina A, Vassylyev DG, Kohno T, Maturana AD, Ito K, Nureki O.
Title Structure and function of a membrane component SecDF that enhances protein export.
Method Solution NMR
Resolution

分子情報[1]

Protein SecDF
Organism Thermus thermophilus
Uniprot Q5SKE6
Sequence P4 domain(470-559)
Ligand
Vector pET-28a
Expression host Escherichia coli (BL21-CodonPlus(DE3)-RIL)
Cell culture growth at 37 deg C, induction with 1 mM IPTG, harvest 16 h at 37 deg C post induction.
Protein purification Mono Q, Superdex 200

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