TP構造ギャラリー
2RRN
PDB ID | 2RRN | |
Protein | SecDF | |
Comment | P4 domain | |
PubMed ID | 21562494 | |
Authors | Tsukazaki T, Mori H, Echizen Y, Ishitani R, Fukai S, Tanaka T, Perederina A, Vassylyev DG, Kohno T, Maturana AD, Ito K, Nureki O. | |
Title | Structure and function of a membrane component SecDF that enhances protein export. | |
Method | Solution NMR | |
Resolution |
分子情報[1]
Protein | SecDF |
Organism | Thermus thermophilus |
Uniprot | Q5SKE6 |
Sequence | P4 domain(470-559) |
Ligand | |
Vector | pET-28a |
Expression host | Escherichia coli (BL21-CodonPlus(DE3)-RIL) |
Cell culture | growth at 37 deg C, induction with 1 mM IPTG, harvest 16 h at 37 deg C post induction. |
Protein purification | Mono Q, Superdex 200 |
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