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TP構造ギャラリー

2RQW

2RQW PDB ID 2RQW
Protein Bem1p SH3CI domain
Comment Ste20p-PRR peptide complex
PubMed ID 20410294
Authors Takaku T, Ogura K, Kumeta H, Yoshida N, Inagaki F.
Title Solution structure of a novel Cdc42 binding module of Bem1 and its interaction with Ste20 and Cdc42.
Method Solution NMR
Resolution

分子情報[1]

Protein Bem1
Organism Saccharomyces cerevisiae
Uniprot P29366
Sequence SH3CI domain(156-260)
Ligand
Vector pGEX-6P-1
Expression host Escherichia coli (BL21(DE3))
Cell culture
Protein purification Glutathione Sepharose 4B, HiLoad 26/60 Superdex 75 pg

分子情報[2]

Protein Ste20
Organism Saccharomyces cerevisiae
Uniprot Q03497
Sequence Bem1-binding domain(463-486)
Ligand
Vector pGEX-6P-1
Expression host Escherichia coli (BL21(DE3))
Cell culture
Protein purification Glutathione Sepharose 4B, RESOURCE RPC

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