TP構造ギャラリー
2RQW
PDB ID | 2RQW | |
Protein | Bem1p SH3CI domain | |
Comment | Ste20p-PRR peptide complex | |
PubMed ID | 20410294 | |
Authors | Takaku T, Ogura K, Kumeta H, Yoshida N, Inagaki F. | |
Title | Solution structure of a novel Cdc42 binding module of Bem1 and its interaction with Ste20 and Cdc42. | |
Method | Solution NMR | |
Resolution |
分子情報[1]
Protein | Bem1 |
Organism | Saccharomyces cerevisiae |
Uniprot | P29366 |
Sequence | SH3CI domain(156-260) |
Ligand | |
Vector | pGEX-6P-1 |
Expression host | Escherichia coli (BL21(DE3)) |
Cell culture | |
Protein purification | Glutathione Sepharose 4B, HiLoad 26/60 Superdex 75 pg |
分子情報[2]
Protein | Ste20 |
Organism | Saccharomyces cerevisiae |
Uniprot | Q03497 |
Sequence | Bem1-binding domain(463-486) |
Ligand | |
Vector | pGEX-6P-1 |
Expression host | Escherichia coli (BL21(DE3)) |
Cell culture | |
Protein purification | Glutathione Sepharose 4B, RESOURCE RPC |
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