TP構造ギャラリー
2RNR
PDB ID | 2RNR | |
Protein | TFIIEalpha acidic domain - TFIIH p62 PH domain complex | |
Comment | ||
PubMed ID | 18354501 | |
Authors | Okuda M, Tanaka A, Satoh M, Mizuta S, Takazawa M, Ohkuma Y, Nishimura Y. | |
Title | Structural insight into the TFIIE-TFIIH interaction: TFIIE and p53 share the binding region on TFIIH. | |
Method | Solution NMR | |
Resolution |
分子情報[1]
Protein | TFIIEalpha |
Organism | Homo sapiens |
Uniprot | P29083 |
Sequence | C-terminal acidic domain(378-439) |
Ligand | |
Vector | pET-3a |
Expression host | Escherichia coli (BL21(DE3)pLysS) |
Cell culture | |
Protein purification | Ni-NTA Agarose, Q Sepharose, Superdex 30 |
分子情報[2]
Protein | TFIIH p62 subunit |
Organism | Homo sapiens |
Uniprot | P32780 |
Sequence | PH domain(1-108) |
Ligand | |
Vector | pGEX-4T-1 |
Expression host | Escherichia coli (BL21(DE3)) |
Cell culture | growth to OD600= 0.6 at 37 deg C, induction with 0.4 mM IPTG, harvest 4 h post induction. |
Protein purification | GSH Sepharose, S15 |
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