TP構造ギャラリー
2LI5
PDB ID | 2LI5 | |
Protein | Atg7-Atg8 complex | |
Comment | Atg7(601-630)-Atg8(K26P) | |
PubMed ID | 22055191 | |
Authors | Noda NN, Satoo K, Fujioka Y, Kumeta H, Ogura K, Nakatogawa H, Ohsumi Y, Inagaki F. | |
Title | Structural basis of Atg8 activation by a homodimeric E1, Atg7. | |
Method | Solution NMR | |
Resolution |
分子情報[1]
Protein | Atg7 |
Organism | Saccharomyces cerevisiae |
Uniprot | P38862 |
Sequence | 601-630 |
Ligand | |
Vector | pACYCDuet-1 |
Expression host | Escherichia coli (BL21) |
Cell culture | |
Protein purification | GST affinity, Ni-chelating, gel filtration |
分子情報[2]
Protein | Atg8 |
Organism | Saccharomyces cerevisiae |
Uniprot | P38182 |
Sequence | K26P |
Ligand | |
Vector | pGEX-6P-1 |
Expression host | Escherichia coli (BL21) |
Cell culture | |
Protein purification | GST affinity, Ni-chelating, gel filtration |
>>一覧に戻る