TP構造ギャラリー
2KWC
PDB ID | 2KWC | |
Protein | Atg8 | |
Comment | K26P mutant | |
PubMed ID | 20428927 | |
Authors | Kumeta H, Watanabe M, Nakatogawa H, Yamaguchi M, Ogura K, Adachi W, Fujioka Y, Noda NN, Ohsumi Y, Inagaki F. | |
Title | The NMR structure of the autophagy-related protein Atg8. | |
Method | Solution NMR | |
Resolution |
分子情報[1]
Protein | Atg8 |
Organism | Saccharomyces cerevisiae |
Uniprot | P38182 |
Sequence | K26P |
Ligand | |
Vector | pGEX-6P-1 |
Expression host | Escherichia coli (BL21) |
Cell culture | induction with 1 mM IPTG, harvest overnight at 25 deg C post induction. |
Protein purification | Glutathione Sepharose 4B, CM, Superdex 75 |
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