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TP構造ギャラリー

2KWC

2KWC PDB ID 2KWC
Protein Atg8
Comment K26P mutant
PubMed ID 20428927
Authors Kumeta H, Watanabe M, Nakatogawa H, Yamaguchi M, Ogura K, Adachi W, Fujioka Y, Noda NN, Ohsumi Y, Inagaki F.
Title The NMR structure of the autophagy-related protein Atg8.
Method Solution NMR
Resolution

分子情報[1]

Protein Atg8
Organism Saccharomyces cerevisiae
Uniprot P38182
Sequence K26P
Ligand
Vector pGEX-6P-1
Expression host Escherichia coli (BL21)
Cell culture induction with 1 mM IPTG, harvest overnight at 25 deg C post induction.
Protein purification Glutathione Sepharose 4B, CM, Superdex 75

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