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TP構造ギャラリー

2E6F

2E6F PDB ID 2E6F
Protein T.cruzi DHOD
Comment oxonate complex
PubMed ID 18808149
Authors Inaoka DK, Sakamoto K, Shimizu H, Shiba T, Kurisu G, Nara T, Aoki T, Kita K, Harada S.
Title Structures of Trypanosoma cruzi dihydroorotate dehydrogenase complexed with substrates and products: atomic resolution insights into mechanisms of dihydroorotate oxidation and fumarate reduction.
Method X-ray diffraction
Resolution 1.26

分子情報[1]

Protein DHOD
Organism Trypanosoma cruzi
Uniprot Q4D3W2
Sequence
Ligand FMN, oxonate
Vector pET-3a
Expression host Escherichia coli (BL21(DE3))
Cell culture growth to OD550= 0.3, induction with 1 mM IPTG, harvest 16 h at 16 deg C post induction.
Protein purification DEAE Sepharose FF, Phenyl Sepharose HP, TSK G3000SW

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