TP構造ギャラリー
2E6D
PDB ID | 2E6D | |
Protein | T.cruzi DHOD | |
Comment | fumarate complex | |
PubMed ID | 18808149 | |
Authors | Inaoka DK, Sakamoto K, Shimizu H, Shiba T, Kurisu G, Nara T, Aoki T, Kita K, Harada S. | |
Title | Structures of Trypanosoma cruzi dihydroorotate dehydrogenase complexed with substrates and products: atomic resolution insights into mechanisms of dihydroorotate oxidation and fumarate reduction. | |
Method | X-ray diffraction | |
Resolution | 1.94 |
分子情報[1]
Protein | DHOD |
Organism | Trypanosoma cruzi |
Uniprot | Q4D3W2 |
Sequence | |
Ligand | FMN, fumarate |
Vector | pET-3a |
Expression host | Escherichia coli (BL21(DE3)) |
Cell culture | growth to OD550= 0.3, induction with 1 mM IPTG, harvest 16 h at 16 deg C post induction. |
Protein purification | DEAE Sepharose FF, Phenyl Sepharose HP, TSK G3000SW |
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