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• Contents
• 1. Overview and objectives of the project
• 2. Membrane proteins and Supramoleclar complexes
• 3. Crystallization of membrane proteins using the cubic liquid crystal method
• 4. Production of membrane proteins by cell-free protein synthesis systems
• 5. Synthesis of large proteins by human cell-free protein synthesis system
• 6. Synthesis of membrane proteins and complexes by living cells
• 7. Site-specific introduction techniques of unnatural amino acids in vivo and in cell-free systems

Non-natural amino acid incorporation into proteins promises to accelerate research activities in protein science.

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