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• Contents
• 1. Overview and objectives of the project
• 2. Membrane proteins and Supramoleclar complexes
• 3. Crystallization of membrane proteins using the cubic liquid crystal method
• 4. Production of membrane proteins by cell-free protein synthesis systems
• 5. Synthesis of large proteins by human cell-free protein synthesis system
• 6. Synthesis of membrane proteins and complexes by living cells
• 7. Site-specific introduction techniques of unnatural amino acids in vivo and in cell-free systems

In life science research, structural analyses of important targets (macromolecular complexes and membrane proteins etc.) frequently encounter severe bottlenecks, because the sample preparation steps are so difficult.

Corresponding to the functions of the samples in vivo, they may undergo membrane embedding, complex formation, or post-translational processing or modification (glycosylation, phosphorylation etc.).

They might have multiple states that enable the regulation of the activity, making the lifetime of the functional structures short. For these reasons, the sample preparation is difficult. In our project, we have developed advanced fundamental technologies that enable the preparation of difficult proteins (macromolecular complexes, membrane proteins etc.).

We will also construct a "structural and functional evaluation system" that can judge whether the samples are suitable for structure analysis.

In addition, we will serve as a basis to prepare samples suitable for structural and functional research, by constructing an "expression screening system," in which many expression protocols and crystallization conditions for difficult target proteins are optimized and systemized.

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