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• Contents
• 1. Overview and objectives of the project
• 2. Membrane proteins and Supramoleclar complexes
• 3. Crystallization of membrane proteins using the cubic liquid crystal method
• 4. Production of membrane proteins by cell-free protein synthesis systems
• 5. Synthesis of large proteins by human cell-free protein synthesis system
• 6. Synthesis of membrane proteins and complexes by living cells
• 7. Site-specific introduction techniques of unnatural amino acids in vivo and in cell-free systems

We have succeeded in synthesizing proteins over 150 kDa by

(i) crushing the membrane and removing unwanted materials from the cultured cells by centrifugation, and

(ii) adding enzymes that enhance the protein production.

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