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• Contents
• 1. Overview and objectives of the project
• 2. Membrane proteins and Supramoleclar complexes
• 3. Crystallization of membrane proteins using the cubic liquid crystal method
• 4. Production of membrane proteins by cell-free protein synthesis systems
• 5. Synthesis of large proteins by human cell-free protein synthesis system
• 6. Synthesis of membrane proteins and complexes by living cells
• 7. Site-specific introduction techniques of unnatural amino acids in vivo and in cell-free systems

Recently, novel crystallization technologies in lipid bilayer matrices, such as "cubic liquid crystal" or "sponge phage," have become the key to crystallize GPCRs.

As the technology is new, many problems still remain.

We are developing a strategical and systematic crystallization method for membrane proteins by

(i) using novel lipid-based "cubic liquid crystal" or "sponge phase" matrices,

(ii) developing devices and methodologies specialized for crystallization, and

(iii) investigating the crystallization mechanism.

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