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Targeted Proteins Research Themes

  • Fundamental Biology
  • Medicine / Pharmacology
  • Food/Environment

Technology Development Themes

  • Protein Production
  • Structural Analysis
  • Chemical Regulation
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TPRP
  • Contents
  • 1. GOAL
  • 2. An anti-GPCR monoclonal antibody P20.1 recognizes 6-residue linear sequence
  • 3. An anti-GPCR monoclonal antibody P20.1 recognizes
  • 4. Binding kinetics analysis by BIAcore
  • 5. Concatenation of P4 sequence dramatically increases affinity
  • 6. Tagged protein purification using P20.1-Sepharose
  • 7. Combination of P20.1 mAb and P43 tag is suitable for recombinant protein purification!
  • 8. Expression vectors carrying P43 tag sequence
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  • Posters on Targeted Proteins Research Program at ICSG 2008
  • Development of novel...

Development of novel affinity tag system for the high-quality production of extracellular and membrane proteins

Principal Investigator: Junichi Takagi

  • 1. GOAL
  • 2. An anti-GPCR monoclonal antibody P20.1 recognizes 6-residue linear sequence
  • 3. An anti-GPCR monoclonal antibody P20.1 recognizes
  • 4. Binding kinetics analysis by BIAcore
  • 5. Concatenation of P4 sequence dramatically increases affinity
  • 6. Tagged protein purification using P20.1-Sepharose
  • 7. Combination of P20.1 mAb and P43 tag is suitable for recombinant protein purification!
  • 8. Expression vectors carrying P43 tag sequence

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