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- Posters on Targeted Proteins Research Program at ICSG 2008
- Development of novel...
Development of novel affinity tag system for the high-quality production of extracellular and membrane proteins
Principal Investigator: Junichi Takagi
- 1. GOAL
- 2. An anti-GPCR monoclonal antibody P20.1 recognizes 6-residue linear sequence
- 3. An anti-GPCR monoclonal antibody P20.1 recognizes
- 4. Binding kinetics analysis by BIAcore
- 5. Concatenation of P4 sequence dramatically increases affinity
- 6. Tagged protein purification using P20.1-Sepharose
- 7. Combination of P20.1 mAb and P43 tag is suitable for recombinant protein purification!
- 8. Expression vectors carrying P43 tag sequence