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3WGC
PDB ID | 3WGC | |
Protein | LATA | |
Comment | H128Y, S292R mutant | |
PubMed ID | 24914980 | |
Authors | Qin HM, Imai FL, Miyakawa T, Kataoka M, Kitamura N, Urano N, Mori K, Kawabata H, Okai M, Ohtsuka J, Hou F, Nagata K, Shimizu S, Tanokura M. | |
Title | L-allo-Threonine aldolase with an H128Y/S292R mutation from Aeromonas jandaei DK-39 reveals the structural basis of changes in substrate stereoselectivity. | |
Method | X-ray diffraction | |
Resolution | 2.50 |
Molecular information[1]
Protein | L-allo-threonine aldolase |
Organism | Aeromonas jandaei |
Uniprot | O07051 |
Sequence | H128Y, S292R |
Ligand | N-glycine-[3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridin-4-yl-methane] |
Vector | pET-28a(+) |
Expression host | Escherichia coli (Rosetta(DE3)) |
Cell culture | growth to OD600= 0.6 at 37 deg C, induction with 0.5 mM IPTG, harvest 4 h at 37 deg C post induction. |
Protein purification | Ni-Sepharose 6FF, RESOURCE Q |