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TP構造ギャラリー

4O0L

4O0L PDB ID 4O0L
Protein RrQR
Comment
PubMed ID 24507746
Authors Takeshita D, Kataoka M, Miyakawa T, Miyazono K, Kumashiro S, Nagai T, Urano N, Uzura A, Nagata K, Shimizu S, Tanokura M.
Title Structural basis of stereospecific reduction by quinuclidinone reductase.
Method X-ray diffraction
Resolution 2.2

分子情報[1]

Protein NADPH-dependent 3-quinuclidinone reductase
Organism Rhodotorula rubra
Uniprot B9ZZZ6
Sequence
Ligand NADPH
Vector pET-28a
Expression host Escherichia coli (Rosetta(DE3))
Cell culture growth to mid-log phase at 25 deg C, induction with 1.0 mM IPTG.
Protein purification Ni-NTA agarose, RESOURCE Q

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