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TP構造ギャラリー

3WXM

3WXM PDB ID 3WXM
Protein Pelota-EF1alpha complex
Comment replaced from 3AGJ
PubMed ID 20876129
Authors Kobayashi K, Kikuno I, Kuroha K, Saito K, Ito K, Ishitani R, Inada T, Nureki O.
Title Structural basis for mRNA surveillance by archaeal Pelota and GTP-bound EF1{alpha} complex.
Method X-ray diffraction
Resolution 2.30

分子情報[1]

Protein EF1alpha
Organism Aeropyrum pernix
Uniprot Q9YAV0
Sequence
Ligand GTP, Mg
Vector pET-15b
Expression host Escherichia coli (BL21-CodonPlus(DE3))
Cell culture growth to OD600= 0.5-0.7 at 37 deg C, induction with 0.5 mM IPTG, harvest overnight at 20 deg C post induction.
Protein purification Ni-NTA Superflow, RESOURCE S, Hiload 16/60 Superdex 200

分子情報[2]

Protein Pelota
Organism Aeropyrum pernix
Uniprot Q9YAZ5
Sequence
Ligand
Vector pET-15b
Expression host Escherichia coli (BL21-CodonPlus(DE3))
Cell culture growth to OD600= 0.5-0.7 at 37 deg C, induction with 0.5 mM IPTG, harvest overnight at 20 deg C post induction.
Protein purification Ni-NTA Superflow, RESOURCE S

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