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TP構造ギャラリー

3WXI

3WXI PDB ID 3WXI
Protein glycerol kinase
Comment
PubMed ID 25315291
Authors Balogun EO, Inaoka DK, Shiba T, Kido Y, Tsuge C, Nara T, Aoki T, Honma T, Tanaka A, Inoue M, Matsuoka S, Michels PA, Kita K, Harada S.
Title Molecular basis for the reverse reaction of African human trypanosomes glycerol kinase.
Method X-ray diffraction
Resolution 2.90

分子情報[1]

Protein glycerol kinase
Organism Trypanosoma brucei gambiense
Uniprot D3KVM3
Sequence
Ligand
Vector pET151/D-TOPO
Expression host Escherichia coli (Jm109(de3+prare2))
Cell culture growth to OD600= 0.6 at 37 deg C, induction with 0.025 mM IPTG, harvest at 20 deg C until late log-phase.
Protein purification Ni-NTA agarose, Superdex 200

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