TP構造ギャラリー
3WXM
PDB ID | 3WXM | |
Protein | Pelota-EF1alpha complex | |
Comment | replaced from 3AGJ | |
PubMed ID | 20876129 | |
Authors | Kobayashi K, Kikuno I, Kuroha K, Saito K, Ito K, Ishitani R, Inada T, Nureki O. | |
Title | Structural basis for mRNA surveillance by archaeal Pelota and GTP-bound EF1{alpha} complex. | |
Method | X-ray diffraction | |
Resolution | 2.30 |
分子情報[1]
Protein | EF1alpha |
Organism | Aeropyrum pernix |
Uniprot | Q9YAV0 |
Sequence | |
Ligand | GTP, Mg |
Vector | pET-15b |
Expression host | Escherichia coli (BL21-CodonPlus(DE3)) |
Cell culture | growth to OD600= 0.5-0.7 at 37 deg C, induction with 0.5 mM IPTG, harvest overnight at 20 deg C post induction. |
Protein purification | Ni-NTA Superflow, RESOURCE S, Hiload 16/60 Superdex 200 |
分子情報[2]
Protein | Pelota |
Organism | Aeropyrum pernix |
Uniprot | Q9YAZ5 |
Sequence | |
Ligand | |
Vector | pET-15b |
Expression host | Escherichia coli (BL21-CodonPlus(DE3)) |
Cell culture | growth to OD600= 0.5-0.7 at 37 deg C, induction with 0.5 mM IPTG, harvest overnight at 20 deg C post induction. |
Protein purification | Ni-NTA Superflow, RESOURCE S |
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