TP構造ギャラリー
3WXI
PDB ID | 3WXI | |
Protein | glycerol kinase | |
Comment | ||
PubMed ID | 25315291 | |
Authors | Balogun EO, Inaoka DK, Shiba T, Kido Y, Tsuge C, Nara T, Aoki T, Honma T, Tanaka A, Inoue M, Matsuoka S, Michels PA, Kita K, Harada S. | |
Title | Molecular basis for the reverse reaction of African human trypanosomes glycerol kinase. | |
Method | X-ray diffraction | |
Resolution | 2.90 |
分子情報[1]
Protein | glycerol kinase |
Organism | Trypanosoma brucei gambiense |
Uniprot | D3KVM3 |
Sequence | |
Ligand | |
Vector | pET151/D-TOPO |
Expression host | Escherichia coli (Jm109(de3+prare2)) |
Cell culture | growth to OD600= 0.6 at 37 deg C, induction with 0.025 mM IPTG, harvest at 20 deg C until late log-phase. |
Protein purification | Ni-NTA agarose, Superdex 200 |
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