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TP構造ギャラリー

3WGC

3WGC PDB ID 3WGC
Protein LATA
Comment H128Y, S292R mutant
PubMed ID 24914980
Authors Qin HM, Imai FL, Miyakawa T, Kataoka M, Kitamura N, Urano N, Mori K, Kawabata H, Okai M, Ohtsuka J, Hou F, Nagata K, Shimizu S, Tanokura M.
Title L-allo-Threonine aldolase with an H128Y/S292R mutation from Aeromonas jandaei DK-39 reveals the structural basis of changes in substrate stereoselectivity.
Method X-ray diffraction
Resolution 2.50

分子情報[1]

Protein L-allo-threonine aldolase
Organism Aeromonas jandaei
Uniprot O07051
Sequence H128Y, S292R
Ligand N-glycine-[3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridin-4-yl-methane]
Vector pET-28a(+)
Expression host Escherichia coli (Rosetta(DE3))
Cell culture growth to OD600= 0.6 at 37 deg C, induction with 0.5 mM IPTG, harvest 4 h at 37 deg C post induction.
Protein purification Ni-Sepharose 6FF, RESOURCE Q

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