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TP構造ギャラリー

3A33

3A33 PDB ID 3A33
Protein UbcH5b~Ubiquitin conjugate
Comment
PubMed ID 20152160
Authors Sakata E, Satoh T, Yamamoto S, Yamaguchi Y, Yagi-Utsumi M, Kurimoto E, Tanaka K., Wakatsuki S, Kato K.
Title Crystal structure of UbcH5b~ubiquitin intermediate: Insight into the formation of the self-assembled E2~Ub conjugates.
Method X-ray diffraction
Resolution 2.2

分子情報[1]

Protein Ubch5b
Organism Homo sapiens
Uniprot P62837
Sequence C85S
Ligand
Vector pET-28a
Expression host Escherichia coli (BL21-CodonPlus(DE3))
Cell culture
Protein purification Ni-NTA affinity, gel filtration, RESOURCE S

分子情報[2]

Protein ubiquitin
Organism Homo sapiens
Uniprot P0CG48
Sequence Gly76 forms covalent bond with Ser85 in Ubch5b.
Ligand
Vector pGEX-6P-1 (Modified)
Expression host Escherichia coli (BL21-CodonPlus(DE3))
Cell culture
Protein purification RESOURCE S

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