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4O0L
PDB ID | 4O0L | |
Protein | RrQR | |
Comment | ||
PubMed ID | 24507746 | |
Authors | Takeshita D, Kataoka M, Miyakawa T, Miyazono K, Kumashiro S, Nagai T, Urano N, Uzura A, Nagata K, Shimizu S, Tanokura M. | |
Title | Structural basis of stereospecific reduction by quinuclidinone reductase. | |
Method | X-ray diffraction | |
Resolution | 2.20 |
Molecular information[1]
Protein | NADPH-dependent 3-quinuclidinone reductase |
Organism | Rhodotorula rubra |
Uniprot | B9ZZZ6 |
Sequence | |
Ligand | NADPH |
Vector | pET-28a |
Expression host | Escherichia coli (Rosetta(DE3)) |
Cell culture | growth to mid-log phase at 25 deg C, induction with 1.0 mM IPTG. |
Protein purification | Ni-NTA agarose, RESOURCE Q |