TP Structure Gallery

3WAE

3WAE PDB ID 3WAE
Protein TtFbpA
Comment Fe, bicarbonate complex
PubMed ID 24419392
Authors Wang S, Ogata M, Horita S, Ohtsuka J, Nagata K, Tanokura M.
Title A novel mode of ferric ion coordination by the periplasmic ferric ion-binding subunit FbpA of an ABC-type iron transporter from Thermus thermophilus HB8.
Method X-ray diffraction
Resolution 1.70

Molecular information[1]

Protein TtFbpA
Organism Thermus thermophilus
Uniprot Q5SHV2
Sequence
Ligand Fe, bicarbonate
Vector pRSFDuet-1
Expression host Escherichia coli (Rosetta(DE3))
Cell culture growth to OD600= 0.6 at 37 deg C, induction with 0.1 mM IPTG, harvest 24 h at 20 deg C post induction.
Protein purification Superdex 200 HR 10/30

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