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3WAE
PDB ID | 3WAE | |
Protein | TtFbpA | |
Comment | Fe, bicarbonate complex | |
PubMed ID | 24419392 | |
Authors | Wang S, Ogata M, Horita S, Ohtsuka J, Nagata K, Tanokura M. | |
Title | A novel mode of ferric ion coordination by the periplasmic ferric ion-binding subunit FbpA of an ABC-type iron transporter from Thermus thermophilus HB8. | |
Method | X-ray diffraction | |
Resolution | 1.70 |
Molecular information[1]
Protein | TtFbpA |
Organism | Thermus thermophilus |
Uniprot | Q5SHV2 |
Sequence | |
Ligand | Fe, bicarbonate |
Vector | pRSFDuet-1 |
Expression host | Escherichia coli (Rosetta(DE3)) |
Cell culture | growth to OD600= 0.6 at 37 deg C, induction with 0.1 mM IPTG, harvest 24 h at 20 deg C post induction. |
Protein purification | Superdex 200 HR 10/30 |