eSOL(Solubility database of all E.coli proteins)

eSOL(Solubility database of all E.coli proteins) is a database on the solubility of entire ensemble E.coli proteins (Niwa T, Ying BW, Saito K, Jin W, Takada S, Ueda T and Taguchi H, 2009) individually synthesized by PURE system that is chaperone free.

Search by gene name

gene name: ade
hit: 5

Result

Table(1)

Table(2)

Chaperone

All

JW_ID	Gene name K-12	Solubility(%)	Calculated MW(kDa)	Gene product description
JW0126	yadE	12	46.3	predicted polysaccharide deacetylase lipoprotein
JW3640	ade	35	63.7	cryptic adenine deaminase
JW5020	fadE	-	89.1	acyl coenzyme A dehydrogenase
JW1729	nadE	-	30.6	NAD synthetase, NH3/glutamine-dependent
JW3480	gadE	-	20.6	DNA-binding transcriptional activator

JW_ID	Gene name K-12	Solubility(%)	Yield(uM)	Minus Sol	TF Sol	GroE Sol	KJE Sol	Minus(uM)	TF(uM)	GroE(uM)	KJE(uM)	Calculated MW(kDa)	Calculated pI	Type of gene product	Gene product description	Cell location	Structure (PDB) id	SCOP assignment
JW0126	yadE	12	1.1	-	-	-	-	-	-	-	-	46.3	10.4	pe	predicted polysaccharide deacetylase lipoprotein	Periplasmic	-	-
JW3640	ade	35	0.4	-	-	-	-	-	-	-	-	63.7	5.5	e	cryptic adenine deaminase	Cytoplasmic	-	51338; Composite domain of metallo-dependent hydrolases
JW5020	fadE	-	-	-	-	-	-	-	-	-	-	89.1	8.2	e	acyl coenzyme A dehydrogenase	Integral Membrane Protein	-	47203; Acyl-CoA dehydrogenase (flavoprotein), C-terminal domain 56645; Acyl-CoA dehydrogenase (flavoprotein), N-terminal and middle domains
JW1729	nadE	-	-	-	-	-	-	-	-	-	-	30.6	6.4	e	NAD synthetase, NH3/glutamine-dependent	Cytoplasmic	-	52402; Adenine nucleotide alpha hydrolases
JW3480	gadE	-	-	-	-	-	-	-	-	-	-	20.6	10.3	r	DNA-binding transcriptional activator	Cytoplasmic	-	46894; C-terminal, effector domain of the bipartite response regulators 52935; Pyruvate kinase, C-terminal domain