eSOL(Solubility database of all E.coli proteins)

eSOL(Solubility database of all E.coli proteins) is a database on the solubility of entire ensemble E.coli proteins (Niwa T, Ying BW, Saito K, Jin W, Takada S, Ueda T and Taguchi H, 2009) individually synthesized by PURE system that is chaperone free.

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Result

gene name: ada
hit: 9

Result

Table(1)

Table(2)

Chaperone

All

JW_ID	Gene name K-12	Solubility(%)	Calculated MW(kDa)	Gene product description
JW0733	nadA	35	38.2	quinolinate synthase, subunit A
JW1178	dadA	12	47.558	D-amino acid dehydrogenase
JW2201	ada	32	39.3	fused DNA-binding transcriptional dual regulator O6-methylguanine-DNA methyltransferase
JW2543	tadA	16	20.014	tRNA-specific adenosine deaminase
JW4092	cadA	5	81.176	lysine decarboxylase 1
JW3485	gadA	62	52.6	glutamate decarboxylase A, PLP-dependent
JW5578	fadA	10	40.837	3-ketoacyl-CoA thiolase (thiolase I)
JW4352	radA	29	49.424	predicted repair protein
JW4291	iadA	-	41	isoaspartyl dipeptidase

JW_ID	Gene name K-12	Solubility(%)	Yield(uM)	Calculated MW(kDa)	Calculated pI	Gene product description	Cell location
JW0733	nadA	35	1.1	38.2	5.7	quinolinate synthase, subunit A	Cytoplasmic
JW1178	dadA	12	0.2	47.558	6.8	D-amino acid dehydrogenase	Cytoplasmic
JW2201	ada	32	3	39.3	8.7	fused DNA-binding transcriptional dual regulator O6-methylguanine-DNA methyltransferase	Cytoplasmic
JW2543	tadA	16	1.1	20.014	8.74	tRNA-specific adenosine deaminase	Cytoplasmic
JW4092	cadA	5	0.2	81.176	6.19	lysine decarboxylase 1	Cytoplasmic
JW3485	gadA	62	0.1	52.6	5.7	glutamate decarboxylase A, PLP-dependent	Cytoplasmic
JW5578	fadA	10	0.8	40.837	6.87	3-ketoacyl-CoA thiolase (thiolase I)	Cytoplasmic
JW4352	radA	29	0.5	49.424	7.46	predicted repair protein	Cytoplasmic
JW4291	iadA	-	-	41	5.6	isoaspartyl dipeptidase	Cytoplasmic

JW_ID	Gene name K-12	Solubility(%)	Calculated MW(kDa)	Type of gene product	Gene product description	Structure (PDB) id	SCOP assignment
JW0733	nadA	35	38.2	e	quinolinate synthase, subunit A	-	-
JW1178	dadA	12	47.558	e	D-amino acid dehydrogenase	-	51971; Nucleotide-binding domain 54373; FAD-linked reductases, C-terminal domain
JW2201	ada	32	39.3	r	fused DNA-binding transcriptional dual regulator O6-methylguanine-DNA methyltransferase	-	46689; Homeodomain-like 57884; N-ada DNA repair protein, N-terminal domain (N-Ada 10) 46767; Methylated DNA-protein cysteine methyltransferase, C-terminal domain 53155; Methylated DNA-protein cysteine methyltransferase domain
JW2543	tadA	16	20.014	e	tRNA-specific adenosine deaminase	-	53927; Cytidine deaminase
JW4092	cadA	5	81.176	e	lysine decarboxylase 1	-	53383; PLP-dependent transferases 55904; Ornithine decarboxylase C-terminal domain
JW3485	gadA	62	52.6	e	glutamate decarboxylase A, PLP-dependent	1PMO	53383; PLP-dependent transferases
JW5578	fadA	10	40.837	e	3-ketoacyl-CoA thiolase (thiolase I)	-	53901; Thiolase-like
JW4352	radA	29	49.424	cp	predicted repair protein	-	48695; Multiheme cytochromes 52540; P-loop containing nucleoside triphosphate hydrolases
JW4291	iadA	-	41	e	isoaspartyl dipeptidase	1POK	51338; Composite domain of metallo-dependent hydrolases

JW_ID	Gene name K-12	Solubility(%)	Minus Sol	TF Sol	GroE Sol	KJE Sol	Minus(uM)	TF(uM)	GroE(uM)	KJE(uM)	Calculated MW(kDa)	Gene product description
JW0733	nadA	35	-	-	-	-	-	-	-	-	38.2	quinolinate synthase, subunit A
JW1178	dadA	12	8.7	29.5	93.45	87.34	0.417	0.404	0.435	0.443	47.558	D-amino acid dehydrogenase
JW2201	ada	32	-	-	-	-	-	-	-	-	39.3	fused DNA-binding transcriptional dual regulator O6-methylguanine-DNA methyltransferase
JW2543	tadA	16	8.97	21.36	33.98	31.01	3.546	2.737	3.942	3.275	20.014	tRNA-specific adenosine deaminase
JW4092	cadA	5	16.76	73.84	102.09	84.69	0.077	0.061	0.007	0.075	81.176	lysine decarboxylase 1
JW3485	gadA	62	-	-	-	-	-	-	-	-	52.6	glutamate decarboxylase A, PLP-dependent
JW5578	fadA	10	8.42	14.39	88.81	31.79	1.702	1.472	1.828	1.431	40.837	3-ketoacyl-CoA thiolase (thiolase I)
JW4352	radA	29	32.09	51.89	96.3	99.7	0.877	1.053	1.437	2.076	49.424	predicted repair protein
JW4291	iadA	-	-	-	-	-	-	-	-	-	41	isoaspartyl dipeptidase

JW_ID	Gene name K-12	Solubility(%)	Yield(uM)	Minus Sol	TF Sol	GroE Sol	KJE Sol	Minus(uM)	TF(uM)	GroE(uM)	KJE(uM)	Calculated MW(kDa)	Calculated pI	Type of gene product	Gene product description	Cell location	Structure (PDB) id	SCOP assignment
JW0733	nadA	35	1.1	-	-	-	-	-	-	-	-	38.2	5.7	e	quinolinate synthase, subunit A	Cytoplasmic	-	-
JW1178	dadA	12	0.2	8.7	29.5	93.45	87.34	0.417	0.404	0.435	0.443	47.558	6.8	e	D-amino acid dehydrogenase	Cytoplasmic	-	51971; Nucleotide-binding domain 54373; FAD-linked reductases, C-terminal domain
JW2201	ada	32	3	-	-	-	-	-	-	-	-	39.3	8.7	r	fused DNA-binding transcriptional dual regulator O6-methylguanine-DNA methyltransferase	Cytoplasmic	-	46689; Homeodomain-like 57884; N-ada DNA repair protein, N-terminal domain (N-Ada 10) 46767; Methylated DNA-protein cysteine methyltransferase, C-terminal domain 53155; Methylated DNA-protein cysteine methyltransferase domain
JW2543	tadA	16	1.1	8.97	21.36	33.98	31.01	3.546	2.737	3.942	3.275	20.014	8.74	e	tRNA-specific adenosine deaminase	Cytoplasmic	-	53927; Cytidine deaminase
JW4092	cadA	5	0.2	16.76	73.84	102.09	84.69	0.077	0.061	0.007	0.075	81.176	6.19	e	lysine decarboxylase 1	Cytoplasmic	-	53383; PLP-dependent transferases 55904; Ornithine decarboxylase C-terminal domain
JW3485	gadA	62	0.1	-	-	-	-	-	-	-	-	52.6	5.7	e	glutamate decarboxylase A, PLP-dependent	Cytoplasmic	1PMO	53383; PLP-dependent transferases
JW5578	fadA	10	0.8	8.42	14.39	88.81	31.79	1.702	1.472	1.828	1.431	40.837	6.87	e	3-ketoacyl-CoA thiolase (thiolase I)	Cytoplasmic	-	53901; Thiolase-like
JW4352	radA	29	0.5	32.09	51.89	96.3	99.7	0.877	1.053	1.437	2.076	49.424	7.46	cp	predicted repair protein	Cytoplasmic	-	48695; Multiheme cytochromes 52540; P-loop containing nucleoside triphosphate hydrolases
JW4291	iadA	-	-	-	-	-	-	-	-	-	-	41	5.6	e	isoaspartyl dipeptidase	Cytoplasmic	1POK	51338; Composite domain of metallo-dependent hydrolases