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VAMP727 compartments target SYP121 foci on the plasma membrane. Root epidermal cells of A. thaliana expressing TagRFP-VAMP727 (red) and GFP-SYP121 (green) were observed by TIRFM. This movie consists of 147 TIRF images acquired every 100 msec. The membrane compartment bearing?@VAMP727 specifically targeted the static SYP121 foci, where they seemed to be tethered, flapping like a flag.