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VAMP727 compartments target SYP121 foci on the plasma membrane. Root epidermal cells of A. thaliana expressing TagRFP-VAMP727 (red) and GFP-SYP121 (green) were observed by TIRFM. This movie consists of 147 TIRF images acquired every 100 msec. The membrane compartment bearing　VAMP727 specifically targeted the static SYP121 foci, where they seemed to be tethered, flapping like a flag.