Whole Cell Project Database -Purification-

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Thermus thermophilus HB8  TTHA0508
transcriptional regulator, MerR family

Lot Information:

Purification Lot: PC010508-42-01-02
sPlasmid ID: PC010508-42
Plasmid Vector: pET-11a
Label: Native
Person: Arima T.
Purified Date: 05/19/2011
Total Protein Yield: 2 mg
Final Volume: 1.3 mL
Conc.: 1.6 mg/mL

Final SDS-PAGE:

Culture Lot:

date

host cell

scale

SDS-PAGE

04/28/2011Rosetta2(DE3)6 L

Cell Lysis and Heat Treatment:

23.3g of E. coli cells
Ultra Sonication with 20mM Tris-HCl + 50mM NaCl (pH8.0)
Heat Treatment at 70°C for 30 min
Ultra Cengrifuge at 200,000G for 1 hr

Purification Procedure:

ColumnBufferFlow Rate (mL/min)Eluted
Resource PHE 6ml50 mM NaPi (pH 7.0), 1.05 M (NH4)2SO45FT
Ammonium sulfate precipitation20 mM Tris-HCl (pH 8.0), 2.5 M (NH4)2SO4  
HiLoad 16/60 Superdex 75 pg50 mM Tris-HCl (pH 9.0), 0.5 M NaCl0.558.24 ml