Whole Cell Project Database -Purification-

Show All

List View

 

Thermus thermophilus HB8  TTHA0255
ferric uptake regulation protein

Lot Information:

Purification Lot: PC010255-41-01-02
sPlasmid ID: PC010255-41
Plasmid Vector: pET-11a
Label: Native
Person: Manzoku M.
Purified Date: 09/30/2005
Total Protein Yield: 2.5 mg
Final Volume: 0.40 mL
Conc.: 6.3 mg/mL

Final SDS-PAGE:

Culture Lot:

date

host cell

scale

SDS-PAGE

07/28/2005BL21(DE3)12 L

Cell Lysis and Heat Treatment:

41.1g of E. coli cells
Ultra Sonication with 20mM Tris-HCl + 50mM NaCl (pH8.0)
Heat Treatment at 70°C for 10 min
Ultra Cengrifuge at 200,000G for 1 hr

Purification Procedure:

ColumnBufferFlow Rate (mL/min)Eluted
Super Q Toyopearl 650M (PACKED)20 mM Tris-HCl (pH 8.0)5270 mM NaCl
Resource Q 6ml20 mM Tris-HCl (pH 8.0)570 mM NaCl
Resource ISO 6ml50 mM NaPi (pH 7.0), 2.5 M (NH4)2SO451.35 M AS
HiLoad 16/60 Superdex 200 pg20 mM Tris-HCl (pH 8.0), 150 mM NaCl0.581 ml