Whole Cell Project Database -Purification-

Show All

List View

 

Thermus thermophilus HB8  TTHA1465
50S ribosomal protein L13

Lot Information:

Purification Lot: PC011465-41-02-04
sPlasmid ID: PC011465-41
Plasmid Vector: pET-11a
Label: SeMet
Person: Kashihara A.
Purified Date: 09/19/2002
Total Protein Yield:   mg
Final Volume:   mL
Conc.:   mg/mL

Final SDS-PAGE:

Culture Lot:

date

host cell

scale

SDS-PAGE

09/13/2002B834(DE3)6 L

Cell Lysis and Heat Treatment:

12.4g of E. coli cells
Ultra Sonication with 20mM Tris-HCl + 50mM NaCl (pH8.0)
Heat Treatment at 60°C for 10 min
Ultra Cengrifuge at 200,000G for 1 hr

Purification Procedure:

ColumnBufferFlow Rate (mL/min)Eluted
Super Q Toyopearl 650M (PACKED)20 mM Tris-HCl (pH 8.0)50.6M NaCl
Resource Q 6ml20 mM Tris-HCl (pH 8.0)50.4M NaCl
HiTrap Heparin 5ml10 mM NaPi (pH 7.0)30.78M NaCl
HiLoad 16/60 Superdex 75 pg20 mM Tris-HCl (pH 8.0), 150 mM NaCl0.5