Whole Cell Project Database -Purification-

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Thermus thermophilus HB8  TTHA1465
50S ribosomal protein L13

Lot Information:

Purification Lot: PC011465-41-02-03
sPlasmid ID: PC011465-41
Plasmid Vector: pET-11a
Label: SeMet
Person: Kashihara A.
Purified Date: 09/06/2002
Total Protein Yield: .5 mg
Final Volume: 0.5 mL
Conc.: 1 mg/mL


Culture Lot:


host cell



08/05/2002B834(DE3)6 L

Cell Lysis and Heat Treatment:

30.2g of E. coli cells
Ultra Sonication with 20mM Tris-HCl + 50mM NaCl (pH8.0)
Heat Treatment at 60°C for 10 min
Ultra Cengrifuge at 200,000G for 1 hr

Purification Procedure:

ColumnBufferFlow Rate (mL/min)Eluted
Super Q Toyopearl 650M (PACKED)20 mM Tris-HCl (pH 8.0)50.3M NaCl
Resource Q 6ml20 mM Tris-HCl (pH 8.0)50.18M NaCl
HiTrap Heparin 5ml10 mM NaPi (pH 7.0)30.64M NaCl
HiLoad 16/60 Superdex 75 pg20 mM Tris-HCl (pH 8.0), 150 mM NaCl0.5