Whole Cell Project Database -Purification-

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Thermus thermophilus HB8  TTHA1969
chromosome partitioning protein, ParB family

Lot Information:

Purification Lot: PC011969-41-00-00
sPlasmid ID: PC011969-41
Plasmid Vector: pET-11a
Label:  
Person:  
Purified Date: 03/31/2000
Total Protein Yield:   mg
Final Volume:   mL
Conc.:   mg/mL

Final SDS-PAGE:

 

Culture Lot:

date

host cell

scale

SDS-PAGE

Cell Lysis and Heat Treatment:

 g of E. coli cells
Ultra Sonication with  
Heat Treatment at  °C for   min
Ultra Cengrifuge at 200,000G for 1 hr

Purification Procedure:

ColumnBufferFlow Rate (mL/min)Eluted