Whole Cell Project Database -Purification-

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Thermus thermophilus HB8  TTHA0812
phosphoribosylamine-glycine ligase, PurD

Lot Information:

Purification Lot: PC010812-41-01-02
sPlasmid ID: PC010812-41
Plasmid Vector: pET-11a
Label: Native
Person: Maoka N.
Purified Date: 06/06/2001
Total Protein Yield: 64 mg
Final Volume: 4.3 mL
Conc.: 14.9 mg/mL

Final SDS-PAGE:

Culture Lot:

date

host cell

scale

SDS-PAGE

05/01/2001BL21 (DE3)2 L

Cell Lysis and Heat Treatment:

10.34g of E. coli cells
Ultra Sonication with  
Heat Treatment at  °C for   min
Ultra Cengrifuge at 200,000G for 1 hr

Purification Procedure:

ColumnBufferFlow Rate (mL/min)Eluted
Resource ISO 6ml50 mM NaPi (pH 7.0), 1.2 M (NH4)2SO45 ml/min0.78 M AS
Resource Q 6ml20 mM Tris-HCl (pH 8.0)5 ml/min0.10 M NaCl
HiLoad 16/60 Superdex 200 pg20 mM Tris-HCl (pH 8.0), 150 mM NaCl0.5ml/min