Whole Cell Project Database -Purification-

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Thermus thermophilus HB8  TTHA0244
single-stranded DNA binding protein

Lot Information:

Purification Lot: PC010244-41-01-01
sPlasmid ID: PC010244-41
Plasmid Vector: pET-11a
Label: Native
Person: Satoh S.
Purified Date: 04/01/2001
Total Protein Yield:   mg
Final Volume:   mL
Conc.:   mg/mL

Final SDS-PAGE:

 

Culture Lot:

date

host cell

scale

SDS-PAGE

Cell Lysis and Heat Treatment:

8.24g of E. coli cells
Ultra Sonication with  
Heat Treatment at  °C for   min
Ultra Cengrifuge at 200,000G for 1 hr

Purification Procedure:

ColumnBufferFlow Rate (mL/min)Eluted
Resource PHE 6ml50 mM NaPi (pH 7.0), 1.05 M (NH4)2SO4  
Resource Q 6ml20 mM Tris-HCl (pH 8.0)  
HiTrap Heparin 5ml20 mM Tris-HCl (pH 8.0)  
Hydroxyapatite CHT10-I10 mM NaPi (pH 7.0)