Whole Cell Project Database -Target Detail-

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Thermus thermophilus HB8  TTHA0062
alanine racemase

Locus: 1:complement(58555..59637)
Synonym: TT1115
DNA Sequence:
(with up/down stream)
Amino Acid Sequence:
Stop Codon: TAG
DNA Length: 1080
AA Length: 360
Mol. Weight: 38657
pI: 10.1
Absorption coefficient: 46515
Number of Methionine: 3

Expression Plasmid:
Plasmid ID:Vector:Description:Met: Distribution from DNA BANK :
PC010062-41pET-11a  not available
PC010062-42pUC18  not available

Purification:
Expression Plasmid:Label:Purified Date:Yield (mg):
PC010062-42Native09/11/200241
PC010062-42Native11/25/200217
PC010062-42Native12/18/200218.2
PC010062-42SeMet04/30/200318.9
PC010062-42Native09/16/20033.2
PC010062-42SeMet09/14/20046.4

Plasmid for Gene Disruption:
BRCID:PDID:Vector:Marker Gene:Upstream:Downstream:
Reference for HTK marker: Hoseki, J. et al. (1999) J Biochem, 126, 951-6.
Reference for gene disruption: Hashimoto, Y. et al. (2001) FEBS Lett, 506, 231-4.

Structure:
PDB_code:Deposit:
the most similar PDB entry:

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