Whole Cell Project Database -Target Detail-

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Thermus thermophilus HB8  TTHA0046
aspartate aminotransferase

Locus: 1:complement(46697..47854)
Synonym: TT0021
DNA Sequence:
(with up/down stream)
Amino Acid Sequence:
Stop Codon: TAG
DNA Length: 1155
AA Length: 385
Mol. Weight: 42056
pI: 6.3
Absorption coefficient: 33495
Number of Methionine: 7

Expression Plasmid:
Plasmid ID:Vector:Description:Met: Distribution from DNA BANK :
PC010046-41pET-11a 7available

Purification:
Expression Plasmid:Label:Purified Date:Yield (mg):

Plasmid for Gene Disruption:
BRCID:PDID:Vector:Marker Gene:Upstream:Downstream:
TDs01A03PD010046-01pGEM-T EasyHTK
Reference for HTK marker: Hoseki, J. et al. (1999) J Biochem, 126, 951-6.
Reference for gene disruption: Hashimoto, Y. et al. (2001) FEBS Lett, 506, 231-4.

Structure:
PDB_code:Deposit:
1GCK08/04/2000
1GC407/19/2000
1GC307/18/2000
5BJ401/11/1999
1B5O01/07/1999
1B5P01/07/1999
1BKG07/07/1998
1BJW06/30/1998
the most similar PDB entry:

Related Articles:
Sakuraba, H. et al., (2008) Structure of an archaeal alanine:glyoxylate aminotransferase Acta Crystallogr D Biol Crystallogr 64 :  696-9
Nakai, T. et al., (1998) Crystallization and preliminary X-ray characterization of aspartate aminotransferase from an extreme thermophile, Thermus thermophilus HB8 Acta Crystallogr D Biol Crystallogr 54 :  1032-4
Okamoto, A. et al., (1996) An aspartate aminotransferase from an extremely thermophilic bacterium, Thermus thermophilus HB8 J Biochem 119 :  135-44