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Thermus thermophilus HB8  TTHA0016
tRNA-dihydrouridine synthase

Locus: 1:17121..18149
Synonym: TT1912
DNA Sequence:
(with up/down stream)
Amino Acid Sequence:
Stop Codon: TAA
DNA Length: 1026
AA Length: 342
Mol. Weight: 38478
pI: 10.1
Absorption coefficient: 26145
Number of Methionine: 10

Expression Plasmid:
Plasmid ID:Vector:Description:Met: Distribution from DNA BANK :
PC010016-10pET-HisTEV 11not available
PC010016-41pET-11a 10available

Purification:
Expression Plasmid:Label:Purified Date:Yield (mg):
PC010016-41SeMet08/08/20023.5
PC010016-41SeMet09/20/20023.3
PC010016-41SeMet02/21/20054.2
PC010016-10Native11/04/20109.4

Plasmid for Gene Disruption:
BRCID:PDID:Vector:Marker Gene:Upstream:Downstream:
TDs01F01PD010016-01pGEM-T EasyHTK
Reference for HTK marker: Hoseki, J. et al. (1999) J Biochem, 126, 951-6.
Reference for gene disruption: Hashimoto, Y. et al. (2001) FEBS Lett, 506, 231-4.

Structure:
PDB_code:Deposit:
the most similar PDB entry:

Related Articles:
Yu, F. et al., (2011) Crystallization and preliminary X-ray crystallographic analysis of dihydrouridine synthase from Thermus thermophilus and its complex with tRNA Acta Crystallogr Sect F Struct Biol Cryst Commun 67 :  685-8