Whole Cell Project Database -Target Detail-

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Thermus thermophilus HB8  TTHA0001
DNA polymerase III, beta subunit

Locus: 1:55..1182
Synonym: TT0744
DNA Sequence:
(with up/down stream)
Amino Acid Sequence:
Stop Codon: TAG
DNA Length: 1125
AA Length: 375
Mol. Weight: 40526
pI: 4.8
Absorption coefficient: 24885
Number of Methionine: 6

Expression Plasmid:
Plasmid ID:Vector:Description:Met: Distribution from DNA BANK :
PC010001-41pET-11a 6available
PC010001-42pET-HisTEV 7not available

Purification:
Expression Plasmid:Label:Purified Date:Yield (mg):
PC010001-41Native07/17/200342.3
PC010001-41SeMet02/24/200434.5
PC010001-41Native12/28/20059.9
PC010001-41SeMet01/12/200618.1
PC010001-41SeMet01/12/200618.1
PC010001-41Native06/12/200721.9
PC010001-42Native01/21/20090

Plasmid for Gene Disruption:
BRCID:PDID:Vector:Marker Gene:Upstream:Downstream:
TDs01A01PD010001-01pGEM-T EasyHTK
Reference for HTK marker: Hoseki, J. et al. (1999) J Biochem, 126, 951-6.
Reference for gene disruption: Hashimoto, Y. et al. (2001) FEBS Lett, 506, 231-4.

Structure:
PDB_code:Deposit:
the most similar PDB entry:

Related Articles:
Bullard, J. M. et al., (2002) DNA polymerase III holoenzyme from Thermus thermophilus identification, expression, purification of components, and use to reconstitute a processive replicase J Biol Chem 277 :  13401-8
McHenry, C. S. et al., (1997) A DNA polymerase III holoenzyme-like subassembly from an extreme thermophilic eubacterium J Mol Biol 272 :  178-89
Ruttimann, C. et al., (1985) DNA polymerases from the extremely thermophilic bacterium Thermus thermophilus HB-8 Eur J Biochem 149 :  41-6