Whole Cell Project Database -Target Detail-

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Thermus thermophilus HB8  TTHA1586
DNA gyrase, subunit B

Locus: 1:1508748..1510652
Synonym: TT0763
DNA Sequence:
(with up/down stream)
Amino Acid Sequence:
Stop Codon: TAA
DNA Length: 1902
AA Length: 634
Mol. Weight: 70533
pI: 6.3
Absorption coefficient: 46830
Number of Methionine: 10

Expression Plasmid:
Plasmid ID:Vector:Description:Met: Distribution from DNA BANK :
PC011586-41pT7Blue 9not available
PC011586-42pET-11a 9not available
PC011586-43pET-HisTEV 11not available

Purification:
Expression Plasmid:Label:Purified Date:Yield (mg):
PC011586-42Native04/30/200435
PC011586-43Native01/23/2009 

Plasmid for Gene Disruption:
BRCID:PDID:Vector:Marker Gene:Upstream:Downstream:
TDs08D08PD011586-01pGEM-T EasyHTK
Reference for HTK marker: Hoseki, J. et al. (1999) J Biochem, 126, 951-6.
Reference for gene disruption: Hashimoto, Y. et al. (2001) FEBS Lett, 506, 231-4.

Structure:
PDB_code:Deposit:
1KIJ12/03/2001
the most similar PDB entry:

Related Articles:
Akanuma, S. et al., (2011) Phylogeny-Based Design of a B-Subunit of DNA Gyrase and Its ATPase Domain Using a Small Set of Homologous Amino Acid Sequences J Mol Biol 412 :  212-25
Lamour, V. et al., (2002) Crystallization of the 43 kDa ATPase domain of Thermus thermophilus gyrase B in complex with novobiocin Acta Crystallogr D Biol Crystallogr 58 :  1376-8
Lamour, V. et al., (2002) An open conformation of the Thermus thermophilus gyrase B ATP-binding domain J Biol Chem 277 :  18947-53