Whole Cell Project Database -Target Detail-

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Thermus thermophilus HB8  TTHB192
CRISPR-associated protein, Cse3

Locus: 2:198797..199432
Synonym: TT1711
DNA Sequence:
(with up/down stream)
Amino Acid Sequence:
Stop Codon: TAG
DNA Length: 633
AA Length: 211
Mol. Weight: 23728
pI: 11
Absorption coefficient: 31815
Number of Methionine: 2

Expression Plasmid:
Plasmid ID:Vector:Description:Met: Distribution from DNA BANK :
PC014192-41pET-11a 2available

Purification:
Expression Plasmid:Label:Purified Date:Yield (mg):
PC014192-41Native07/23/200316.1
PC014192-41Native01/24/20059
PC014192-41Native06/07/20056.1

Plasmid for Gene Disruption:
BRCID:PDID:Vector:Marker Gene:Upstream:Downstream:
TDs11E04PD014192-01pGEM-T EasyHTK
Reference for HTK marker: Hoseki, J. et al. (1999) J Biochem, 126, 951-6.
Reference for gene disruption: Hashimoto, Y. et al. (2001) FEBS Lett, 506, 231-4.

Structure:
PDB_code:Deposit:
1WJ905/29/2004
the most similar PDB entry:

Related Articles:
Kondo, N. et al., (2009) Thermus thermophilus-derived protein tags that aid in preparation of insoluble viral proteins Anal Biochem 385 :  278-85
Ebihara, A. et al., (2006) Crystal structure of hypothetical protein TTHB192 from Thermus thermophilus HB8 reveals a new protein family with an RNA recognition motif-like domain Protein Sci 15 :  1494-9